Anti-Human CD30 Ligand/TNFSF8 Rabbit Recombinant Antibody

CD30 Ligand/TNFSF8 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241831D9

Cat no : 98253-1-RR

Synonyms

TNFSF 8, CD30 L, TNFSF8, 241831D9, CD30LG

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Validation Data Gallery

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  FC experiment of human PBMCs using 98253-1-RR

  1x10^6 untreated or PMA and ionomycin treated human PBMCs were surface stained with 0.25 ug Anti-Human CD30 Ligand/TNFSF8 Rabbit Recombinant Antibody (98253-1-RR, Clone: 241831D9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3). Cells were not fixed.

• 

  FC experiment of human PBMCs using 98253-1-RR

  1x10^6 PMA and ionomycin treated human PBMCs were surface stained with 0.25 ug Anti-Human CD30 Ligand/TNFSF8 Rabbit Recombinant Antibody (98253-1-RR, Clone: 241831D9) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human PBMCs were surface stained with 0.25 ug Anti-Human CD30 Ligand/TNFSF8 Rabbit Recombinant Antibody (98253-1-RR, Clone: 241831D9) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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Tested Applications

• Positive FC detected in: PMA and ionomycin treated human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98253-1-RR targets CD30 Ligand/TNFSF8 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: tumor necrosis factor (ligand) superfamily, member 8
• Calculated Molecular Weight: 234 aa, 26 kDa
• GenBank Accession Number: BC093630
• Gene Symbol: TNFSF8
• Gene ID (NCBI): 944
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CD30 Ligand (CD30L), also named as TNFSF8 or CD153, is a type II transmembrane protein that belongs to the tumor necrosis factor (TNF) superfamily (PMID: 8391931). It is expressed on the surface of activated T cells, B cells, monocytes, macrophages, eosinophils, neutrophils, and mast cells (PMID: 26090498). CD30L interacts with its receptor, CD30, which is a cell surface protein expressed on a small subset of activated T and B lymphocytes, and a variety of lymphoid neoplasms, with the highest expression in classical Hodgkin lymphoma and anaplastic large cell lymphomas (PMID: 28885612). The CD30/CD30L signaling is involved in pleiotropic downstream effects including differentiation, cell survival and death, NFkB activation, and production of cytokines (PMID: 38534788).