CoraLite® Plus 488-conjugated CD3 Gamma Monoclonal antibody

CD3 Gamma Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2E9G7

Cat no : CL488-60347

Synonyms

CD3 GAMMA, CD3G, T cell receptor T3 gamma chain, T3G

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Validation Data Gallery

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  IF Staining of human tonsillitis using CL488-60347

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL488-60347 (CD3 gamma antibody) at dilution of 1:100.

• 

  IF Staining of human tonsillitis using CL488-60347

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL488-60347 (CD3 gamma antibody) at dilution of 1:100.

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Tested Applications

• Positive IF-P detected in: human tonsillitis tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-60347 targets CD3 Gamma in IF-P applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD3 Gamma fusion protein Ag15502
• Full Name: CD3g molecule, gamma (CD3-TCR complex)
• Calculated Molecular Weight: 182 aa, 20 kDa
• GenBank Accession Number: BC113830
• Gene Symbol: CD3 Gamma
• Gene ID (NCBI): 917
• RRID: AB_2883146
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells. The antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.