CD3 Gamma Monoclonal antibody

CD3 Gamma Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

CloneNo.

2E9G7

Cat no : 60347-1-Ig

Synonyms

CD3 gamma, CD3G, T cell receptor T3 gamma chain, T3G

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Validation Data Gallery

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  WB analysis of Jurkat using 60347-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60347-1-Ig (CD3 gamma antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 60347-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60347-1-Ig (CD3 gamma antibody at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

• 

  IHC staining of human tonsillitis using 60347-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60347-1-Ig (CD3 gamma antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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Tested Applications

• Positive WB detected in: Jurkat cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

60347-1-Ig targets CD3 Gamma in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD3 Gamma fusion protein Ag15502
• Full Name: CD3g molecule, gamma (CD3-TCR complex)
• Calculated Molecular Weight: 182 aa, 20 kDa
• Observed Molecular Weight: 20-25 kDa
• GenBank Accession Number: BC113830
• Gene Symbol: CD3 Gamma
• Gene ID (NCBI): 917
• RRID: AB_2881456
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells.

Publications

Species	Application	Title
human	IHC	Cancer Sci; Metformin changes the immune microenvironment of colorectal cancer in patients with type 2 diabetes mellitus.; Authors - Akira Saito
human	IF	J Breast Cancer; Metformin Alters Tumor Immune Microenvironment, Improving the Outcomes of Breast Cancer Patients With Type 2 Diabetes Mellitus; Authors - Satomi Shiba
human	IHC	J Anus Rectum Colon; Reduced Abundance of Phocaeicola in Mucosa-associated Microbiota Is Associated with Distal Colorectal Cancer Metastases Possibly through an Altered Local Immune Environment; Authors - Gaku Ota