CoraLite® Plus 647 Anti-Human CD3 (SK7)
CD3 Monoclonal Antibody for FC
Host / Isotype
Mouse / IgG1, kappa
Reactivity
Human
Applications
FC
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
SK7
Cat no : CL647-65148
Synonyms
Validation Data Gallery
Tested Applications
| Positive FC detected in | human peripheral blood lymphocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL647-65148 targets CD3 in FC applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | N/A |
| Full Name | CD3e molecule, epsilon (CD3-TCR complex) |
| Calculated Molecular Weight | 207 aa, 23 kDa |
| GenBank Accession Number | BC049847 |
| Gene Symbol | CD3 |
| Gene ID (NCBI) | 916 |
| RRID | AB_2920263 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 647 CD3 antibody CL647-65148 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Sareena (Verified Customer) (08-09-2023) | Performed well with compensation beads
|
FH Sareena (Verified Customer) (07-28-2023) | worked well in duplex stain
|
FH Sunil (Verified Customer) (07-28-2023) | Worked great as part of a panel for staining comp beads
|
