Anti-Human CD3 (SK7)

CD3 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

SK7

Cat no : 65148-1-Ig

Synonyms

CD3E, CD3 epsilon, T-cell surface antigen T3/Leu-4 epsilon chain, T3E, SK7

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Validation Data Gallery

• 

  FC experiment of human PBMCs using 65148-1-Ig

  1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD3 (65148-1-Ig, Clone:SK7) (red) or Mouse IgG1 Isotype Control (65124-1-Ig, Clone: MOPC-21) (blue) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM005). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65148-1-Ig

  1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD3 (65148-1-Ig, Clone:SK7) (red) or Mouse IgG1 Isotype Control (65124-1-Ig, Clone: MOPC-21) (blue) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM005). Cells were not fixed. Lymphocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65148-1-Ig targets CD3 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: CD3e molecule, epsilon (CD3-TCR complex)
• Calculated Molecular Weight: 207 aa, 23 kDa
• GenBank Accession Number: BC049847
• Gene Symbol: CD3
• Gene ID (NCBI): 916
• RRID: AB_2918433
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).