CoraLite®568 Anti-Mouse CD3 (17A2)

CD3 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Mouse and More (1)

Applications

FC

Conjugate

CoraLite®568 Fluorescent Dye

Publications

1

CloneNo.

17A2

Cat no : CL568-65077

Synonyms

AI504783, CD3, Cd3e, CD3epsilon, T3e

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Validation Data Gallery

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  FC experiment of mouse splenocytes using CL568-65077

  1X10^6 mouse splenocytes were surface stained with 5 ul CoraLite® Plus 647 Anti-Mouse CD4 (CL647-65104, Clone: GK1.5) and 5 ul CoraLite®568 Anti-Mouse CD3 (CL568-65077, Clone:17A2). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using CL568-65077

  1X10^6 mouse splenocytes were surface stained with 5 ul CoraLite® Plus 647 Anti-Mouse CD4 (CL647-65104, Clone: GK1.5) and CoraLite®568-Rat IgG2b isotype control. Cells were not fixed.

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 1 publications below

Product Information

CL568-65077 targets CD3 in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Cited Reactivity: mouse
• Host / Isotype: Rat / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: γδTCR-positive T-T hybridoma D1
• Full Name: CD3 antigen, epsilon polypeptide
• GenBank Accession Number: BC098236
• Gene Symbol: CD3ε
• Gene ID (NCBI): 12501
• RRID: AB_2919716
• Conjugate: CoraLite®568 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 568 nm / 587 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

Publications

Species	Application	Title
mouse	FC	Acta Pharm Sin B; Novel Pt(IV) complex OAP2 induces STING activation and pyroptosis via mitochondrial membrane remodeling for synergistic chemo-immunotherapy; Authors - Renming Fan