Anti-Mouse CD3 (17A2)

CD3 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

17A2

Cat no : 65077-1-Ig

Synonyms

AI504783, CD3, Cd3e, CD3epsilon, T3e

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Validation Data Gallery

• 

  FC experiment of C57BL/6 mouse splenocytes using 65077-1-Ig

  1x10^6 C57BL/6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD3 (17A2) (65077-1-Ig, Clone:17A2) or 0.5 ug Rat IgG2b Isotype Control (LTF-2) (65211-1-Ig, Clone: LTF-2) and FITC anti-Rat IgG2b Antibody, and 0.5 ug CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5) (CL647-65104, Clone: GK1.5). Cells were not fixed.

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Tested Applications

• Positive FC detected in: C57BL/6 mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65077-1-Ig targets CD3 in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rat / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: γδTCR-positive T-T hybridoma D1
• Full Name: CD3 antigen, epsilon polypeptide
• GenBank Accession Number: BC098236
• Gene Symbol: CD3ε
• Gene ID (NCBI): 12501
• RRID: AB_2918381
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).