CoraLite® Plus 488-conjugated CD3 Monoclonal antibody

CD3 Monoclonal Antibody for IF/ICC, IF-P

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC, IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3F3A1

Cat no : CL488-60181

Synonyms

CD3E, T-cell surface antigen T3/Leu-4 epsilon chain, T3E, CD3-epsilon, CD3 epsilon

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Validation Data Gallery

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  IF Staining of human lung cancer using CL488-60181

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite® Plus 488 CD3 antibody (CL488-60181, Clone: 3F3A1 ) at dilution of 1:100, CoraLite®555 pan-keratin antibody (CL555-26411, orange), CoraLite® Plus 647 CD20 antibody (CL647-60271, Clone: 4A7G3, Magenta).

• 

  IF Staining of human lung cancer using CL488-60181

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite® Plus 488 CD3 antibody (CL488-60181, Clone: 3F3A1 ) at dilution of 1:100, CoraLite®555 pan-keratin antibody (CL555-26411, orange), CoraLite® Plus 647 CD20 antibody (CL647-60271, Clone: 4A7G3, Magenta).

• 

  IF Staining of human tonsillitis using CL488-60181

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488 CD3 antibody (CL488-60181, Clone: 3F3A1 ) at dilution of 1:100, CoraLite®594 human IgM antibody (CL594-66484, Clone: 2D10B10, red), CoraLite® Plus 647 human IgD antibody (CL647-16489, Magenta).

• 

  IF Staining of human tonsillitis using CL488-60181

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488 CD3 antibody (CL488-60181, Clone: 3F3A1 ) at dilution of 1:100, CoraLite®594 human IgM antibody (CL594-66484, Clone: 2D10B10, red), CoraLite® Plus 647 human IgD antibody (CL647-16489, Magenta).

• 

  IF Staining of Jurkat using CL488-60181

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CoraLite® Plus 488 CD3 antibody (CL488-60181, Clone: 3F3A1 ) at dilution of 1:100.

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Tested Applications

• Positive IF-P detected in: human lung cancer tissue, human tonsillitis tissue
• Positive IF/ICC detected in: Jurkat cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-60181 targets CD3 in IF/ICC, IF-P applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD3 fusion protein Ag11797
• Full Name: CD3e molecule, epsilon (CD3-TCR complex)
• Calculated Molecular Weight: 207 aa, 23 kDa
• GenBank Accession Number: BC049847
• Gene Symbol: CD3
• Gene ID (NCBI): 916
• RRID: AB_2883113
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells. This anti-CD3 is a monoclonal antibody directed against the epsilon chain of human CD3 molecule. And the antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.