Biotin Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody

CD3 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

FC

Conjugate

Biotin

CloneNo.

OKT3

Cat no : Biotin-65569

Synonyms

CD3E, T3E, TCRE

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Validation Data Gallery

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  FC experiment of human PBMCs using Biotin-65569

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin CD3 Recombinant Antibody (Biotin-65569, Clone: OKT3) or 0.25 ug Isotype Control, and CoraLite®488-conjugated streptavidin. Cells were then stained with CoraLite® Plus 405 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using Biotin-65569

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin CD3 Recombinant Antibody (Biotin-65569, Clone: OKT3) and CoraLite®488-conjugated streptavidin (red) or 0.25 ug Isotype Control and CoraLite®488-conjugated streptavidin (blue). Cells were not fixed. Lymphocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-65569 targets CD3 in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: Human T cells
• Full Name: CD3e molecule, epsilon (CD3-TCR complex)
• Calculated Molecular Weight: 207 aa, 23 kDa
• GenBank Accession Number: BC049847
• Gene Symbol: CD3
• Gene ID (NCBI): 916
• Conjugate: Biotin
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).