CD3 Recombinant antibody, PBS Only

• Featured Product
• KD/KO Validated

CD3 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4I19

Cat no : 81324-1-PBS

Synonyms

CD3E, T-cell surface antigen T3/Leu-4 epsilon chain, T3E, CD3-epsilon, CD3 epsilon

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Validation Data Gallery

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  WB analysis of Jurkat using 81324-1-RR (same clone as 81324-1-PBS)

  WB result of CD3 antibody (81324-1-RR; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD3 transfected Jurkat cells. This data was developed using the same antibody clone with 81324-1-PBS in a different storage buffer formulation.

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  WB analysis using 81324-1-RR (same clone as 81324-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81324-1-RR (CD3E antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81324-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 81324-1-RR (same clone as 81324-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 81324-1-RR (CD3 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81324-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 81324-1-RR (same clone as 81324-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 81324-1-RR (CD3 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81324-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 81324-1-RR (same clone as 81324-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 81324-1-RR (CD3 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81324-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 81324-1-RR (same clone as 81324-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 81324-1-RR (CD3 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81324-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse small intestine using 81324-1-RR (same clone as 81324-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 81324-1-RR (CD3 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81324-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse small intestine using 81324-1-RR (same clone as 81324-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 81324-1-RR (CD3 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81324-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 81324-1-RR (same clone as 81324-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 81324-1-RR (CD3 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81324-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 81324-1-RR (same clone as 81324-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 81324-1-RR (CD3 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81324-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse spleen using 81324-1-RR (same clone as 81324-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD3 antibody (81324-1-RR, Clone: 4I19 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81324-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 81324-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81324-1-RR against Human CD3 were performed. The affinity constant is 1.13 nM.

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Product Information

81324-1-PBS targets CD3 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CD3 fusion protein Ag11797
• Full Name: CD3e molecule, epsilon (CD3-TCR complex)
• Calculated Molecular Weight: 207 aa, 23 kDa
• Observed Molecular Weight: 23-25 kDa
• GenBank Accession Number: BC049847
• Gene Symbol: CD3
• Gene ID (NCBI): 916
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells. This antibody is directed against the epsilon chain of human CD3 molecule.