Biotin Anti-Human CD28 Rabbit Recombinant Antibody

• 

  FC experiment of human PBMCs using Biotin-98032

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD28 Rabbit Recombinant Antibody (Biotin-98032, Clone: 240354D2) and CoraLite®488-conjugated streptavidin, or unstained. Cells were then stained with CoraLite® Plus 647 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using Biotin-98032

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD28 Rabbit Recombinant Antibody (Biotin-98032, Clone:240354D2) and CoraLite®488-conjugated streptavidin (red), or unstained (blue). Cells were not fixed. Lymphocytes were gated.

CD28 Uni-rAb^TM Recombinant Antibody for FC

Cat No. Biotin-98032

Clone No.240354D2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

Tp44, T-cell-specific surface glycoprotein CD28, CD28 molecule, 240354D2

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-98032 targets CD28 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: CD28 molecule
• Calculated Molecular Weight: 220 aa, 25 kDa
• GenBank Accession Number: BC093698
• Gene Symbol: CD28
• Gene ID (NCBI): 940
• ENSEMBL Gene ID: ENSG00000178562
• RRID: AB_3672378
• Conjugate: Biotin
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P10747
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD28 (T-cell-specific surface glycoprotein CD28), also known as T44 and Tp44, is a 44 kD disulfide-linked homodimeric type I glycoprotein (PMID: 2162180). It is a member of the immunoglobulin superfamily and is expressed on most T lineage cells, NK cell subsets, and plasma cells (PMID: 2162180, 8386518). CD28 may affect in vivo immune responses by functioning both as a cell adhesion molecule linking B and T lymphocytes and as the surface component of a novel signal transduction pathway (PMID: 2162180, 3021470). CD28 binds both CD80 and CD86 with a highly conserved motif MYPPY in the CDR3-like loop (PMID: 15696168, 7964482). CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death (PMID: 1348520).