Anti-Human CD28 Rabbit Recombinant Antibody, PBS Only

CD28 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

240354D2

Cat no : 98032-1-PBS

Synonyms

Tp44, T-cell-specific surface glycoprotein CD28, CD28 molecule, 240354D2

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Validation Data Gallery

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  FC experiment of human PBMCs using 98032-1-RR (same clone as 98032-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD28 Rabbit Recombinant Antibody (98032-1-RR, Clone: 240354D2) or Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cell were then stained with CoraLite® Plus 647 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 98032-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 98032-1-RR (same clone as 98032-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD28 Rabbit Recombinant Antibody (98032-1-RR, Clone: 240354D2) (red) or Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 98032-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 98032-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98032-1-RR against Human CD28 were performed. The affinity constant is 3.23 nM.

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Product Information

The immunogen of 98032-1-PBS is CD28 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: CD28 molecule
• Calculated Molecular Weight: 220 aa, 25 kDa
• GenBank Accession Number: BC093698
• Gene Symbol: CD28
• Gene ID (NCBI): 940
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.