CD28 Recombinant antibody, PBS Only

CD28 Uni-rAb^TM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240354D2

Cat no : 83383-6-PBS

Synonyms

CD28, CD28 molecule, Tp44

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Validation Data Gallery

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  IF Staining of Jurkat using 83383-6-RR (same clone as 83383-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells and THP-1 cells (negative control) using CD28 antibody (83383-6-RR, Clone: 240354D2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83383-6-PBS in a different storage buffer formulation.

• 

  IF Staining of Jurkat using 83383-6-RR (same clone as 83383-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells and THP-1 cells (negative control) using CD28 antibody (83383-6-RR, Clone: 240354D2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83383-6-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83383-6-PBS

  Biolayer interferometry (BLl) kinetic assays of 83383-6-RR against Human CD28 were performed. The affinity constant is 1.99 nM.

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Product Information

The immunogen of 83383-6-PBS is CD28 Fusion Protein expressed in E. coli.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: CD28 molecule
• Calculated Molecular Weight: 220 aa, 25 kDa
• GenBank Accession Number: BC093698
• Gene Symbol: CD28
• Gene ID (NCBI): 940
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.