FITC Plus Anti-Mouse PD-1/CD279 (RMP1-30)

PD-1/CD279 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Mouse

Applications

FC

Conjugate

FITC Plus Fluorescent Dye

CloneNo.

RMP1-30

Cat no : FITC-65142

Synonyms

CD279, Ly101, PD 1, PD-1, Pdc1, Pdcd1, programmed cell death 1

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Validation Data Gallery

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  FC experiment of BALB/c mouse splenocytes using FITC-65142

  1X10^6 Con-A stimulated BALB/c mouse splenocytes were surface stained with 0.5 ug FITC Plus Anti-Mouse PD-1/CD279 (FITC-65142, Clone: RMP1-30) or isotype control antibody. Cells were not fixed.

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Tested Applications

• Positive FC detected in: Con-A stimulated BALB/c mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

FITC-65142 targets PD-1/CD279 in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rat / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Mouse PD-1 transfected BHK cells
• Full Name: programmed cell death 1
• GenBank Accession Number: BC119179
• Gene Symbol: Pdcd1
• Gene ID (NCBI): 18566
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436).