PE Anti-Mouse PD-1/CD279 (J43.1)

PD-1/CD279 Monoclonal Antibody for FC

Host / Isotype

Armenian Hamster / IgG

Reactivity

Mouse

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

J43.1

Cat no : PE-65120

Synonyms

CD279, Ly101, PD 1, PD-1, Pdc1, Pdcd1, programmed cell death 1

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Validation Data Gallery

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  FC experiment of mouse splenocytes using PE-65120

  1X10^6 unstimulated and anti-CD3/CD28-stimulated (3 days) mouse splenocytes were surface co-stained with APC Anti-Mouse CD3 and 0.5 ug PE Anti-Mouse PD-1/CD279 (PE-65120, Clone: J43.1). Cells were not fixed.

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Tested Applications

• Positive FC detected in: anti-CD3/CD28-stimulated mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

PE-65120 targets PD-1/CD279 in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Armenian Hamster / IgG
• Class: Monoclonal
• Type: Antibody
• Immunogen: B12 cells, mouse PD-1 cDNA-transfected BHK cells
• Full Name: programmed cell death 1
• GenBank Accession Number: BC119179
• Gene Symbol: Pdcd1
• Gene ID (NCBI): 18566
• RRID: AB_2883908
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436).