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B7-H3 Monoclonal antibody

B7-H3 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Pig and More (1)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

1E7D1

Cat no : 66481-1-Ig

Synonyms

4Ig B7 H3, B7 homolog 3, B7-H3, CD276, CD276 antigen, CD276 molecule, Costimulatory molecule, UNQ309/PRO352



Tested Applications

Positive WB detected inHEK-293 cells, A431 cells, PC-3 cells
Positive IHC detected inhuman tonsillitis tissue, human placenta tissue, human prostate cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue
Positive FC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66481-1-Ig targets B7-H3 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with Human, Pig samples.

Tested Reactivity Human, Pig
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen B7-H3 fusion protein Ag6006
Full Name CD276 molecule
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC062581
Gene Symbol CD276
Gene ID (NCBI) 80381
RRIDAB_2881847
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

B7-H3 (CD276) is a type I transmembrane protein expressed on many tissues and cell types. B7-H3 is a 100-kDa glycoprotein that belongs to the B7 immunoregulatory family and participates in the regulation of T-cell-mediated immune response probably by functioning as both a T cell costimulator and coinhibitor (PMID: 25567370; 20696859). Overexpressed on a wide range of human solid cancers, B7-H3 has been implicated in cancer progression and metastasis and becomes an attractive target for cancer immunotherapy (PMID: 27208063).

Protocols

Product Specific Protocols
WB protocol for B7-H3 antibody 66481-1-IgDownload protocol
IHC protocol for B7-H3 antibody 66481-1-IgDownload protocol
IF protocol for B7-H3 antibody 66481-1-IgDownload protocol
FC protocol for B7-H3 antibody 66481-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,FC

J Immunother Cancer

Genome-scale CRISPR-Cas9 screen reveals novel regulators of B7-H3 in tumor cells.

Authors - Shasha Zhao
humanIP

J Transl Med

An N-glycoproteomic site-mapping analysis reveals glycoprotein alterations in esophageal squamous cell carcinoma.

Authors - Yingzhen Gao
humanWB,IHC

J Cancer Res Clin Oncol

B7-H3 and CD47 co-expression in gastric cancer is a predictor of poor prognosis and potential targets for future dual-targeting immunotherapy

Authors - Siji Chen
humanWB,CoIP

J Cancer

B7-H3 inhibits apoptosis of gastric cancer cell by interacting with Fibronectin.

Authors - Meiyun Sun
humanIHC

Int J Gen Med

Comprehensive Analysis Reveals Distinct Immunological and Prognostic Characteristics of CD276/B7-H3 in Pan-Cancer

Authors - Jinye Ding
humanWB,IHC,CoIP

FEBS Open Bio

Fibronectin enhances tumor metastasis through B7-H3 in clear cell renal cell carcinoma

Authors - Jinjing Xie