B7-H3 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

B7-H3 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E7D1

Cat no : 66481-1-PBS

Synonyms

CD276, B7H3, B7 homolog 3, B7 H3, 4Ig-B7-H3

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Validation Data Gallery

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  WB analysis of HEK-293 using 66481-1-Ig (same clone as 66481-1-PBS)

  WB result of B7-H3/CD276 antibody (66481-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with normal HEK-293 (sh-control ) and three independent sh-B7-H3 transfected HEK-293 cells. This data was developed using the same antibody clone with 66481-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66481-1-Ig (same clone as 66481-1-PBS)

  HEK-293, PC-3, LNCaP, HeLa cells, and human spleen, human placenta, pig spleen tissues were subjected to SDS PAGE followed by western blot with 66481-1-Ig (B7-H3/CD276 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66481-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 66481-1-Ig (same clone as 66481-1-PBS)

  Untreated and PNGase F-treated lysates of A431 cells and PC-3 cells were subjected to SDS PAGE followed by western blot with 66481-1-Ig (B7-H3/CD276 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 66481-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66481-1-Ig (same clone as 66481-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66481-1-Ig (B7-H3/CD276 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66481-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66481-1-Ig (same clone as 66481-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66481-1-Ig (B7-H3/CD276 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66481-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66481-1-Ig (same clone as 66481-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66481-1-Ig (B7-H3/CD276 antibody) at dilution of 1:5000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66481-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 66481-1-Ig (same clone as 66481-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66481-1-Ig (B7-H3/CD276 antibody at dilution of 1:750 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66481-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 66481-1-Ig (same clone as 66481-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66481-1-Ig (B7-H3/CD276 antibody at dilution of 1:750 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66481-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66481-1-Ig (same clone as 66481-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66481-1-Ig (B7-H3/CD276 antibody at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66481-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66481-1-Ig (same clone as 66481-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66481-1-Ig (B7-H3/CD276 antibody at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66481-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66481-1-Ig (same clone as 66481-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66481-1-Ig (B7-H3/CD276 antibody at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66481-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66481-1-Ig (same clone as 66481-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66481-1-Ig (B7-H3/CD276 antibody at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66481-1-PBS in a different storage buffer formulation.

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  IF Staining of human placenta using 66481-1-Ig (same clone as 66481-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using B7-H3/CD276 antibody (66481-1-Ig, Clone: 1E7D1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66481-1-PBS in a different storage buffer formulation.

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Product Information

66481-1-PBS targets B7-H3 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: B7-H3 fusion protein Ag6006
• Full Name: CD276 molecule
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC062581
• Gene Symbol: CD276
• Gene ID (NCBI): 80381
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

B7-H3 (CD276) is a type I transmembrane protein expressed on many tissues and cell types. B7-H3 is a 100-kDa glycoprotein that belongs to the B7 immunoregulatory family and participates in the regulation of T-cell-mediated immune response probably by functioning as both a T cell costimulator and coinhibitor (PMID: 25567370; 20696859). Overexpressed on a wide range of human solid cancers, B7-H3 has been implicated in cancer progression and metastasis and becomes an attractive target for cancer immunotherapy (PMID: 27208063).