Anti-Human TWEAKR/CD266 Rabbit Recombinant Antibody

CD266 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

240991C5

Cat no : 98125-1-RR

Synonyms

TNFRSF12A, TweakR, FGF-inducible 14, FGF inducible 14, DR3LG

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Validation Data Gallery

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  FC experiment of HT-1080 using 98125-1-RR

  1x10^6 HT-1080 cells were surface stained with 0.25 ug Anti-Human TWEAKR Rabbit Recombinant Antibody (98125-1-RR, Clone: 240991C5) (red) or 0.25 ug Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98125-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98125-1-RR against Human CD266 were performed. The affinity constant is 0.508 nM.

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Tested Applications

• Positive FC detected in: HT-1080 cells

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98125-1-RR is CD266 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CD266 fusion protein Ag13823
• Full Name: tumor necrosis factor receptor superfamily, member 12A
• Calculated Molecular Weight: 129 aa, 14 kDa
• GenBank Accession Number: BC002718
• Gene Symbol: TWEAKR
• Gene ID (NCBI): 51330
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

TweakR (Fn14, TNFRSF12A) is a member of the TNF receptor superfamily which is activated by its ligand, the cytokine TWEAK (TNFSF12). TweakR is the smallest member of the TNFR superfamily . TweakR was first described as an FGF-inducible gene which played a role in fibroblast adhesion and migration . Subsequently, the induction of TweakR expression by other growth factors and/or upon tissue injury was observed in multiple cell types, including hepatocytes, endothelial cells, adipocytes, and cardiomyocytes. (PMID: 23073510, PMID: 24409185)