CoraLite® Plus 750 Anti-Human CD244 Rabbit Recombinant Antibody

• 

  FC experiment of human PBMCs using CL750-98133

  1x10^6 human PBMCs were surface stained with CoraLite® Plus 488 Anti-Human CD3, and 5 ul CoraLite® Plus 750 Anti-Human CD244 Rabbit RecAb (CL750-98133, Clone: 241260G10) or CoraLite® Plus 750 Rabbit IgG Isotype Control RecAb (CL750-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using CL750-98133

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 750 Anti-Human CD244 Rabbit RecAb (CL750-98133, Clone:241260G10) (red), or CoraLite® Plus 750 Rabbit IgG Isotype Control RecAb (CL750-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

CD244 Uni-rAb^TM Recombinant Antibody for FC

Cat No. CL750-98133

Clone No.241260G10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Synonyms

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is N/A per 10^6 cells in a 100 µl suspension or N/A per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL750-98133 targets CD244 in applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: CD244 molecule, natural killer cell receptor 2B4
• Calculated Molecular Weight: 365 aa, 41 kDa
• GenBank Accession Number: BC053985
• Gene Symbol: CD244
• Gene ID (NCBI): 51744
• Conjugate: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 755 nm / 780 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9BZW8
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD244, also known as SLAMF4 or 2B4, is a type I transmembrane glycoprotein belonging to the signaling lymphocyte activation molecule (SLAM) family. It consists of an extracellular segment with two immunoglobulin (Ig)-like domains, a transmembrane region, and a cytoplasmic domain containing tyrosine-based motifs (PMID: 9841922; 30546369). CD244 is present on natural killer (NK) cells, γδ T cells, a subset of CD8+ T cells, monocytes, basophils, dendritic cells, and myeloid-derived suppressor cells (MDSCs) (PMID: 30546369). It binds to CD48 with high affinity and transmits stimulatory or inhibitory signals that regulate immune function (PMID: 9841922; 18523281).