CD24 Monoclonal antibody, PBS Only

• 

  WB analysis using 67627-1-Ig (same clone as 67627-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67627-1-Ig (CD24 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67627-1-PBS in a different storage buffer formulation.

• 

  IF Staining of Daudi using 67627-1-Ig (same clone as 67627-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Daudi cells using CD24 antibody (67627-1-Ig, Clone: 1H5C4 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 67627-1-PBS in a different storage buffer formulation.

CD24 Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 67627-1-PBS

Clone No.1H5C4

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

CD24A, 1H5C4

---

Product Information

67627-1-PBS targets CD24 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD24 fusion protein Ag11679
• Full Name: CD24 molecule
• Calculated Molecular Weight: 8 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC007674
• Gene Symbol: CD24
• Gene ID (NCBI): 100133941
• RRID: AB_2882828
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P25063
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD24 (known as heat stable antigen) is a small highly glycosylated GPI-linked sialoprotein. It is normally expressed at the surface of most B lymphocytes and differentiating neuroblasts, and it is also up-regulated in a wide variety of cancers. Studies have shown that CD24 functions in the regulation of B-cell apoptosis, leukocyte signal transduction, and leukocyte adhesion. Since it is highly glycosylated, the apparent molecular weight of CD24 could be variable, ranging from 30 kDa to 70 kDa. (Ref: Akihiko Sano, MD., 2009)