CD226 Recombinant antibody, PBS Only

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  WB analysis of human peripheral blood platelets using 82991-3-RR (same clone as 82991-3-PBS)

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 82991-3-RR (CD226 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82991-3-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 82991-3-RR (same clone as 82991-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82991-3-RR (CD226 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82991-3-PBS in a different storage buffer formulation.

• 

  IF Staining of HEL using 82991-3-RR (same clone as 82991-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEL cells using CD226 antibody (82991-3-RR, Clone: 230041E12 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82991-3-PBS in a different storage buffer formulation.

CD226 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 82991-3-PBS

Clone No.230041E12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

DNAM-1, DNAM1, DNAM 1, CD226 molecule, CD226 antigen

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Product Information

82991-3-PBS targets CD226 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: CD226 molecule
• Calculated Molecular Weight: 336 aa, 39 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC074787
• Gene Symbol: CD226
• Gene ID (NCBI): 10666
• ENSEMBL Gene ID: ENSG00000150637
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q15762
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD226 (DNAM-1) is a ~65 kDa glycoprotein expressed on the surface of NK cells, platelets, monocytes and a subset of T cells. It is a member of the Ig-superfamily containing 2 Ig-like domains of the V-set. CD226 mediates cellular adhesion of platelets and megakaryocytic cells to vascular endothelial cells. The protein also plays a role in megakaryocytic cell maturation. Interactions of CD226 and its ligands, CD155 and CD112, induce NK and T cell-mediated cytotoxicity and cytokine secretion (PMID: 15039383).