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CD226 Recombinant antibody

CD226 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230041E12

Cat no : 82991-3-RR

Synonyms

DNAM-1, DNAM1, DNAM 1, CD226 molecule, CD226 antigen

Validation Data Gallery

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  • Western Blot (WB) analysis of human peripheral blood platelets using CD226 Recombinant antibody (82991-3-RR)

    WB analysis of human peripheral blood platelets using 82991-3-RR

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 82991-3-RR (CD226 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 82991-3-RR

    Affinity and Kinetic Characterization of 82991-3-RR

    Biolayer interferometry (BLl) kinetic assays of 82991-3-RR against Human CD226 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inhuman peripheral blood platelets

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 82991-3-RR is CD226 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name CD226 molecule
Calculated Molecular Weight 336 aa, 39 kDa
Observed Molecular Weight65 kDa
GenBank Accession NumberBC074787
Gene Symbol CD226
Gene ID (NCBI) 10666
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD226 (DNAM-1) is a ~65 kDa glycoprotein expressed on the surface of NK cells, platelets, monocytes and a subset of T cells. It is a member of the Ig-superfamily containing 2 Ig-like domains of the V-set. CD226 mediates cellular adhesion of platelets and megakaryocytic cells to vascular endothelial cells. The protein also plays a role in megakaryocytic cell maturation. Interactions of CD226 and its ligands, CD155 and CD112, induce NK and T cell-mediated cytotoxicity and cytokine secretion (PMID: 15039383).

Protocols

Product Specific Protocols
WB protocol for CD226 antibody 82991-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols