CoraLite® Plus 750-conjugated CD22 Recombinant antibody
CD22 Recombinant Antibody for WB
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB
Conjugate
CoraLite® Plus 750 Fluorescent Dye
CloneNo.
230550C11
Cat no : CL750-83303
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Raji cells, Daudi cells, Ramos cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:8000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
The immunogen of CL750-83303 is CD22 Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | Fusion Protein |
Full Name | CD22 molecule |
Calculated Molecular Weight | 95kd |
Observed Molecular Weight | 130 kDa |
GenBank Accession Number | NM_001771.4 |
Gene Symbol | CD22 |
Gene ID (NCBI) | 933 |
Conjugate | CoraLite® Plus 750 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 755 nm / 780 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CD22, also known as Siglec-2 (sialic acid binding Ig-like lectin 2) or BL-CAM (B-lymphocyte cell adhesion molecule), is a 130-140 kDa, B-cell restricted, type I transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. The expression of CD22 is developmentally regulated. It is expressed at low levels in the cytoplasm of pro-B and pre-B cells and present on the cell surface only at mature stages of B-cell differentiation. Cell surface expression is lost during terminal differentiation into plasma cell and after B-cell activation. CD22 is an inhibitory receptor for B-cell receptor (BCR) signalling, preferentially binds to alpha-2,6-linked sialic acid and mediates B-cell B-cell interactions. It plays a crucial role in activation and differentiation of the B-cell.
Protocols
Product Specific Protocols | |
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WB protocol for CL Plus 750 CD22 antibody CL750-83303 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |