CoraLite® Plus 555 Anti-Human CD21 (BU32)
CD21 Monoclonal Antibody for FC
Host / Isotype
Mouse / IgG1, kappa
Reactivity
Human
Applications
FC
Conjugate
CoraLite® Plus 555 Fluorescent Dye
CloneNo.
BU32
Cat no : CL555-65198
Synonyms
Validation Data Gallery
Tested Applications
Positive FC detected in | human PBMCs |
Recommended dilution
Application | Dilution |
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This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL555-65198 targets CD21 in FC applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Mouse / IgG1, kappa |
Class | Monoclonal |
Type | Antibody |
Immunogen | N/A |
Full Name | complement component (3d/Epstein Barr virus) receptor 2 |
Calculated Molecular Weight | 1092 aa, 119 kDa |
GenBank Accession Number | BC136394 |
Gene Symbol | CD21 |
Gene ID (NCBI) | 1380 |
Conjugate | CoraLite® Plus 555 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 554 nm / 570 nm |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA. |
Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD21, also known as complement receptor type 2 (CR2), complement C3d receptor, and Epstein-Barr virus receptor, is a transmembrane protein that contains a small cytoplasmic domain, a transmembrane region and an extracellular domain consisting of 15 tandem short consensus repeat sequences (PMID: 6230668; 2551147). It is expressed on B cells, follicular dendritic cells, thymocytes and a subset of peripheral T cells (PMID: 26119182). CD21 binds complement fragments C3d, C3dg and iC3b and acts as a receptor for the Epstein-Barr virus (PMID: 7753047). On B cells, together with CD19 and CD81, CD21 forms a complex that functions as a co-receptor to the BCR (PMID: 7542009). It is involved in B cells activation and functions.
Protocols
Product Specific Protocols | |
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FC protocol for CL Plus 555 CD21 antibody CL555-65198 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |