CD21/CD35 Recombinant antibody

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  WB analysis using 84565-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84565-2-RR (CD21/CD35 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 84565-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84565-2-RR (CD21 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 84565-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84565-2-RR (CD21 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 84565-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84565-2-RR (CD21 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84565-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84565-2-RR against Mouse CD21/CD35 were performed. The affinity constant is 4.84 nM.

CD21/CD35 {ptg:Brand} Recombinant Antibody for WB, IHC, ELISA

Cat No. 84565-2-RR

Clone No.241957E6

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, ELISA

Synonyms

CD21, Cr2, 241957E6

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Tested Applications

• Positive WB detected in: A20 cells, mouse spleen tissue, rat spleen tissue
• Positive IHC detected in: mouse spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84565-2-RR targets CD21/CD35 in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: complement receptor 2
• Calculated Molecular Weight: 113kDa
• Observed Molecular Weight: 160-210 kDa
• GenBank Accession Number: AAA37448.1
• Gene Symbol: Cr2
• Gene ID (NCBI): 12902
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P19070
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD21, also known as complement receptor type 2 (CR2), complement C3d receptor, and Epstein-Barr virus receptor, is a transmembrane protein that contains a small cytoplasmic domain, a transmembrane region and an extracellular domain consisting of 15 short consensus repeats (SCRs) (PMID: 6230668; 2551147). CD21 binds complement fragments C3d, C3dg and iC3b and acts as a receptor for the Epstein-Barr virus (PMID: 7753047). CD35, also known as complement receptor type 1 (CR1) or C3b/C4b receptor, binds C3b and C4b. The murine Cr2 gene encodes for both CD21 and CD35 (PMID: 2139460; 19559484). Mouse CD35 comprises 21 SCRs while mouse CD21 is identical to mouse CD35 except that it lacks the first 6 SCRs. CD21/CD35 forms trimolecular complexes with CD19 and CD81 and plays roles in the regulation of humoral immune responses (PMID: 11043778).