CD209 Recombinant antibody

CD209 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240907A2

Cat no : 84095-6-RR

Synonyms

DC SIGN1, DC SIGN, C-type lectin domain family 4 member L, CLEC4L, CDSIGN

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Validation Data Gallery

• 

  WB analysis of human placenta using 84095-6-RR

  human placenta tissue was subjected to SDS PAGE followed by western blot with 84095-6-RR (CD209 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84095-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84095-6-RR against Human CD209 were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: human placenta tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84095-6-RR is CD209 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: CD209 molecule
• Calculated Molecular Weight: 404 aa, 45.7 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC110615
• Gene Symbol: CD209
• Gene ID (NCBI): 30835
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD209 (DC-SIGN, CLEC4L) is a C-type lectin receptor that is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens, including bacteria, viruses, and parasites. CD209 is preferentially expressed on dendritic cells (DCs). It mediates transient adhesion of DCs with T cells. It has been reported that CD209 plays a role in capture and transmission of HIV from DC to T cells (PMID: 10721995).