CoraLite® Plus 647-conjugated CD20 Monoclonal antibody

CD20 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

4A7G3

Cat no : CL647-60271

Synonyms

B lymphocyte antigen CD20, B1, Bp35, CD20, LEU 16, MS4A1, MS4A1,CD20, MS4A2, S7

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Validation Data Gallery

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  IF Staining of human lung cancer using CL647-60271

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite® Plus 647 CD20 antibody (CL647-60271, Clone: 4A7G3 ) at dilution of 1:100, CoraLite®488 CD3 antibody (CL488-60181, Clone: 3F3A1, green), CoraLite®555 pan-keratin antibody (CL555-26411, orange).

• 

  IF Staining of human lung cancer using CL647-60271

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite® Plus 647 CD20 antibody (CL647-60271, Clone: 4A7G3 ) at dilution of 1:100, CoraLite®488 CD3 antibody (CL488-60181, Clone: 3F3A1, green), CoraLite®555 pan-keratin antibody (CL555-26411, orange).

• 

  IF Staining of human lung cancer using CL647-60271

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite® Plus 647 CD20 antibody (CL647-60271, Clone: 4A7G3 ) at dilution of 1:100, CoraLite®488 pan-keratin antibody (CL488-26411, green).

• 

  IF Staining of human tonsillitis using CL647-60271

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL647-60271 (CD20 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive IF detected in: human lung cancer tissue, human tonsillitis tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-60271 targets CD20 in IF applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD20 fusion protein Ag17309
• Full Name: membrane-spanning 4-domains, subfamily A, member 1
• Calculated Molecular Weight: 297 aa, 33 kDa
• GenBank Accession Number: BC002807
• Gene Symbol: CD20
• Gene ID (NCBI): 931
• RRID: AB_2883651
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CD20 is a 33-37 kDa transmembrane phosphoprotein. CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells. CD20 functions as calcium-permeable cation channel. It is involved in the regulation of B-cell activation and proliferation. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B-cells.