CoraLite®594 Anti-Human CD2 (TS1/8)

CD2 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

TS1/8

Cat no : CL594-65192

Synonyms

LFA-3 receptor, LFA-2, LFA 3 receptor, LFA 2, Erythrocyte receptor

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Validation Data Gallery

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  FC experiment of human PBMCs using CL594-65192

  1x10^6 human PBMCs were surface stained with CL750 Anti-Human CD3 and 5 ul CoraLite®594 Anti-Human CD2 (TS1/8) (CL594-65192) or CoraLite®594 Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using CL594-65192

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite®594 Anti-Human CD2 (TS1/8) (CL594-65192) or CoraLite®594 Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-65192 targets CD2 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: CD2 molecule
• Calculated Molecular Weight: 351 aa, 39 kDa
• GenBank Accession Number: BC033583
• Gene Symbol: CD2
• Gene ID (NCBI): 914
• RRID: AB_2883471
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light.

Background Information

CD2 is a cell surface glycoprotein present on a majority of thymocytes, all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.