CoraLite® Plus 405 Anti-Human CD2 (TS1/8) Mouse IgG2a Recombinant Antibody

CD2 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

CoraLite® Plus 405 Fluorescent Dye

CloneNo.

TS1/8

Cat no : CL405-65565

Synonyms

CD2, LFA-2, LFA 3 receptor, LFA 2, Erythrocyte receptor

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Validation Data Gallery

• 

  FC experiment of human PBMCs using CL405-65565

  1x10^6 human PBMCs were surface stained with CoraLite® Plus 647 Anti-Human CD19, and 5 ul CoraLite® Plus 405 Anti-Human CD2 (TS1/8) Mouse IgG2a Recombinant Antibody (CL405-65565, Clone: TS1/8) (right), or CoraLite® Plus 405 Mouse IgG2a Isotype Control (C1.18.4) (CL405-65208, Clone: C1.18.4) (left). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using CL405-65565

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 405 Anti-Human CD2 (TS1/8) Mouse IgG2a Recombinant Antibody (CL405-65565, Clone: TS1/8)(red), or CoraLite® Plus 405 Mouse IgG2a Isotype Control (C1.18.4) (CL405-65208, Clone: C1.18.4) (blue). Cells were not fixed. Lymphocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL405-65565 targets CD2 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: N/A
• Full Name: CD2 molecule
• Calculated Molecular Weight: 351 aa, 39 kDa
• GenBank Accession Number: BC033583
• Gene Symbol: CD2
• Gene ID (NCBI): 914
• Conjugate: CoraLite® Plus 405 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 399 nm / 422 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD2 is a cell surface glycoprotein present on a majority of thymocytes, all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.