FC experiment of mouse splenocytes using CL488-65242
1x10^6 mouse splenocytes were surface stained with 0.5 ug CoraLite® Plus 488 Anti-Mouse CD1d (1B1) (CL488-65242, Clone:1B1) or 0.5 ug CoraLite® Plus 488 Rat IgG2b Isotype Control (LTF-2) (CL488-65211, Clone: LTF-2). Cells were co-stained with Anti-Mouse CD45R (B220). Cells were not fixed.
1x10^6 mouse splenocytes were surface stained with 0.5 ug CoraLite® Plus 488 Anti-Mouse CD1d (1B1) (CL488-65242, Clone:1B1) or 0.5 ug CoraLite® Plus 488 Rat IgG2b Isotype Control (LTF-2) (CL488-65211, Clone: LTF-2). Cells were co-stained with Anti-Mouse CD45R (B220). Cells were not fixed.
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This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
CL488-65242 targets CD1d in FC applications and shows reactivity with mouse samples.
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
Background Information
CD1d is a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. CD1d is an antigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on NKT cells. When activated, NKT cells rapidly produce Th1 and Th2 cytokines.
Protocols
Product Specific Protocols
FC protocol for CL Plus 488 CD1d antibody CL488-65242
FC experiment of mouse splenocytes using CL488-65242
1x10^6 mouse splenocytes were surface stained with 0.5 ug CoraLite® Plus 488 Anti-Mouse CD1d (1B1) (CL488-65242, Clone:1B1) or 0.5 ug CoraLite® Plus 488 Rat IgG2b Isotype Control (LTF-2) (CL488-65211, Clone: LTF-2). Cells were co-stained with Anti-Mouse CD45R (B220). Cells were not fixed.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
CD1d Monoclonal antibody
Catalog Number
CL488-65242
Citations
-
Dilutions
Applications
FC
Reactivity
mouse
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
