Anti-Mouse CD197 (4B12)

CD197 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

4B12

Cat no : 65158-1-Ig

Synonyms

Ccr7, CD197, Cdw197, Cmkbr7, EBI1, Ebi1h

---

Validation Data Gallery

• 

  FC experiment of mouse splenocytes using 65158-1-Ig

  1X10^6 mouse splenocytes were surface stained with 5 ul CoraLite® Plus 647-conjugated Anti-Mouse CD3 (CL647-65077, Clone: 17A2), and 1 ug Anti-Mouse CD197 (65158-1-Ig, Clone: 4B12) and FITC Anti-Rat IgG2a Antibody at dilution 1:200. Cells were not fixed.

• 

  FC experiment of mouse splenocytes using 65158-1-Ig

  1X10^6 mouse splenocytes were surface stained with 5 ul CoraLite® Plus 647-conjugated Anti-Mouse CD3 (CL647-65077, Clone: 17A2), and 1 ug Rat IgG2a Isotype Control and FITC Anti-Rat IgG2a Antibody at dilution 1:200. Cells were not fixed.

---

Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65158-1-Ig targets CD197 in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Mouse CCR7 Transfected Cell Line
• Full Name: chemokine (C-C motif) receptor 7
• Gene Symbol: CD197
• Gene ID (NCBI): 12775
• RRID: AB_2918440
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Purified by protein-G affinity chromatography
• Storage Buffer: PBS with 0.1% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.