Cardinal Red™ Anti-Human CD163 (GHI/61)

• 

  FC experiment of human PBMCs using CR-65169

  1X10^6 human PBMCs were surface stained with 5 ul Cardinal Red™ Anti-Human CD163 (CR-65169, Clone:GHI/61) (red) or unstained. Cells were not fixed. Monocytes and granulocytes were gated.

• 

  FC experiment of human PBMCs using CR-65169

  1X10^6 human PBMCs were surface stained with 5 ul Cardinal Red™ Anti-Human CD163 (CR-65169, Clone:GHI/61) or unstained. Cells were not fixed. Monocytes and granulocytes were gated.

CD163 Monoclonal Antibody for FC

Cat No. CR-65169

Clone No.GHI/61

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Synonyms

CD163, CD163 molecule, Hemoglobin scavenger receptor, M130, MM130, sCD163

---

Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in 100 μl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

CR-65169 targets CD163 in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Glycoprotein preparation from hairy cell leukemia spleen

• Full Name: CD163 molecule
• Calculated Molecular Weight: 1156 aa, 125 kDa
• GenBank Accession Number: BC051281
• Gene Symbol: CD163
• Gene ID (NCBI): 9332
• ENSEMBL Gene ID: ENSG00000177575
• RRID: AB_3084970
• Conjugate: Cardinal Red™ Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 592 nm / 611 nm
• Form: Liquid
• Purification Method: Purified by protein-A affinity chromatography
• UNIPROT ID: Q86VB7
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD163, also known as M130, is a membrane glycoprotein which belongs to the scavenger receptor superfamily (PMID: 8370408). It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages (PMID: 11196644). CD163 mediates endocytosis of haptoglobin-haemoglobin complexes (PMID: 11196644). The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response (PMID: 22900885). Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity (PMID: 12570164).