CoraLite®594-conjugated CD163 Polyclonal antibody

• Featured Product
• KD/KO Validated

CD163 Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF-P

Conjugate

CoraLite®594 Fluorescent Dye

Publications

2

Cat no : CL594-16646

Synonyms

CD163, CD163 molecule, Hemoglobin scavenger receptor, M130, MM130, sCD163

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Validation Data Gallery

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  IF Staining of human tonsillitis using CL594-16646

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD163 antibody (CL594-16646) at dilution of 1:200.

• 

  IF Staining of human tonsillitis using CL594-16646

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD163 antibody (CL594-16646) at dilution of 1:200.

• 

  IF Staining of human liver using CL594-16646

  Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CoraLite®594 CD163 antibody (CL594-16646) at dilution of 1:200.

• 

  IF Staining of human liver using CL594-16646

  Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CoraLite®594 CD163 antibody (CL594-16646) at dilution of 1:200.

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Tested Applications

• Positive IF-P detected in: human tonsillitis tissue, human liver tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

CL594-16646 targets CD163 in WB, IF-P applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD163 fusion protein Ag10029
• Full Name: CD163 molecule
• Calculated Molecular Weight: 1156 aa, 125 kDa
• GenBank Accession Number: BC051281
• Gene Symbol: CD163
• Gene ID (NCBI): 9332
• RRID: AB_2919836
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CD163 is a transmembrane protein which belongs to the scavenger receptor cysteine-rich (SRCR) superfamily. This protein is a scavenger receptor for the hemoglobin-haptoglobin complex and is a marker for monocytes and macrophages. Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity.

Publications

Species	Application	Title
human	IF	Front Immunol; Identification of CD101 in Glioma: A Novel Prognostic Indicator Expressed on M2 Macrophages.; Authors - Yuyang Liu
human	WB	Phytomedicine; Ginsenoside RK3 inhibits glioblastoma by modulating macrophage M2 polarization via the PPARG/CCL2 axis; Authors - Haiying Zhang