CoraLite® Plus 488 Anti-Human CD163 (GHI/61)

CD163 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human and More (1)

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Publications

2

CloneNo.

GHI/61

Cat no : CL488-65169

Synonyms

CD163, CD163 molecule, Hemoglobin scavenger receptor, M130, MM130, sCD163

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Validation Data Gallery

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  FC experiment of human PBMCs using CL488-65169

  1x10^6 human PBMCs were surface stained with CL750 Anti-Human CD3 and 5 ul CoraLite® Plus 488 Anti-Human CD163 (CL488-65169, Clone:GHI/61) or CoraLite® Plus 488 Mouse IgG1 Isotype Control (CL488-65124). Cells were treated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes and monocytes were gated.

• 

  FC experiment of human PBMCs using CL488-65169

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD163 (CL488-65169, Clone:GHI/61) or CoraLite® Plus 488 Mouse IgG1 Isotype Control (CL488-65124). Cells were treated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes and monocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 2 publications below

Product Information

CL488-65169 targets CD163 in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Glycoprotein preparation from hairy cell leukemia spleen
• Full Name: CD163 molecule
• Calculated Molecular Weight: 1156 aa, 125 kDa
• GenBank Accession Number: BC051281
• Gene Symbol: CD163
• Gene ID (NCBI): 9332
• RRID: AB_2883212
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD163, also known as M130, is a membrane glycoprotein which belongs to the scavenger receptor superfamily (PMID: 8370408). It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages (PMID: 11196644). CD163 mediates endocytosis of haptoglobin-haemoglobin complexes (PMID: 11196644). The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response (PMID: 22900885). Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity (PMID: 12570164).

Publications

Species	Application	Title
human	FC	Oncol Lett; Tumor-associated macrophage-derived exosomes promote EGFR-TKI resistance in non-small cell lung cancer by regulating the AKT, ERK1/2 and STAT3 signaling pathways; Authors - Shiyang Yuan
human	FC	Cell Mol Life Sci; Tumor-associated macrophage-derived exosomal miR21-5p promotes tumor angiogenesis by regulating YAP1/HIF-1α axis in head and neck squamous cell carcinoma; Authors - Quan Yan