CD163 Monoclonal antibody, PBS Only

CD163 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G7A5

Cat no : 68218-1-PBS

Synonyms

CD163, CD163 molecule, Hemoglobin scavenger receptor, M130, MM130, sCD163

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Validation Data Gallery

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  WB analysis of human placenta using 68218-1-Ig (same clone as 68218-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 68218-1-Ig (CD163 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68218-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 68218-1-Ig (same clone as 68218-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68218-1-Ig (CD163 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68218-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 68218-1-Ig (same clone as 68218-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68218-1-Ig (CD163 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68218-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 68218-1-Ig (same clone as 68218-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68218-1-Ig (CD163 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68218-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 68218-1-Ig (same clone as 68218-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68218-1-Ig (CD163 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68218-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 68218-1-Ig (same clone as 68218-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68218-1-Ig (CD163 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68218-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 68218-1-Ig (same clone as 68218-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68218-1-Ig (CD163 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68218-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 68218-1-Ig (same clone as 68218-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68218-1-Ig (CD163 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68218-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 68218-1-Ig (same clone as 68218-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68218-1-Ig (CD163 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68218-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 68218-1-Ig (same clone as 68218-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 68218-1-Ig (CD163 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68218-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human appendicitis using 68218-1-Ig (same clone as 68218-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 68218-1-Ig (CD163 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68218-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human liver using 68218-1-Ig (same clone as 68218-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CD163 antibody (68218-1-Ig, Clone: 2G7A5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68218-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human liver using 68218-1-Ig (same clone as 68218-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CD163 antibody (68218-1-Ig, Clone: 2G7A5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68218-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68218-1-PBS targets CD163 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD163 fusion protein Ag26721
• Full Name: CD163 molecule
• Calculated Molecular Weight: 1156 aa, 125 kDa
• Observed Molecular Weight: 145 kDa
• GenBank Accession Number: BC051281
• Gene Symbol: CD163
• Gene ID (NCBI): 9332
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD163 is a transmembrane protein which belongs to the scavenger receptor cysteine-rich (SRCR) superfamily. This protein is a scavenger receptor for the hemoglobin-haptoglobin complex and is a marker for monocytes and macrophages. Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity.