FITC Anti-Human CD161 (HP-3G10)

CD161 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

FITC Fluorescent Dye

CloneNo.

HP-3G10

Cat no : FITC-65115

Synonyms

CD161, CLEC5B, hNKR P1A, KLRB1, NKR, NKR P1, NKR P1A, NKRP1A

---

Validation Data Gallery

• 

  FC experiment of human peripheral blood lymphocytes using FITC-65115

  1X10^6 human peripheral blood lymphocytes were surface stained with 0.50 ug APC Anti-Human CD4 APC-65143, Clone: RPA-T4) and 0.50 ug FITC Anti-Human CD161 (FITC-65115, Clone: HP-3G10). Cells were not fixed.

• 

  FC experiment of human peripheral blood lymphocytes using FITC-65115

  1X10^6 human peripheral blood lymphocytes were surface stained with 0.50 ug APC Anti-Human CD4 (APC-65143, Clone: RPA-T4) and 0.50 ug FITC-mouse IgG1 isotype control. Cells were not fixed.

---

Tested Applications

• Positive FC detected in: human peripheral blood lymphocytes

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

FITC-65115 targets CD161 in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Human NK cells
• Full Name: killer cell lectin-like receptor subfamily B, member 1
• Calculated Molecular Weight: 225 aa, 25 kDa
• GenBank Accession Number: BC114516
• Gene Symbol: KLRB1
• Gene ID (NCBI): 3820
• RRID: AB_2883785
• Conjugate: FITC Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 498 nm / 526 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD161, also known as KLRB1 or NKR-P1A, is a type II transmembrane C-type lectin-like receptor and is expressed on the cell membrane as disulfide-linked homodimer (PMID: 8077657). It is expressed by the majority of NK cells and subsets of peripheral T cells, including both CD4+ and CD8+ T cells, and is expressed preferentially on adult T cells with a "memory" antigenic phenotype (PMID: 8077657; 22566826). Expression of CD161 correlates with the cytotoxic function of CD16+ NK cells, and ligation of CD161 with its ligand LLT1 inhibits NK cell cytotoxicity and cytokine secretion (PMID: 29686665).