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APC Anti-Human CD4 (RPA-T4)

CD4 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

APC Fluorescent Dye

CloneNo.

RPA-T4

Cat no : APC-65143

Synonyms

CD4, CD4 molecule, CD4mut

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using APC Anti-Human CD4 (RPA-T4) (APC-65143)

    FC experiment of human PBMCs using APC-65143

    1X10^6 human PBMCs were surface co-stained with FITC Anti-Human CD3 and 5 ul APC Anti-Human CD4 (APC-65143, Clone:RPA-T4) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using APC Anti-Human CD4 (RPA-T4) (APC-65143)

    FC experiment of human PBMCs using APC-65143

    1X10^6 human PBMCs were surface stained with 5 ul APC Anti-Human CD4 (APC-65143, Clone:RPA-T4) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

APC-65143 targets CD4 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen N/A
Full Name CD4 molecule
Calculated Molecular Weight 55 kDa
GenBank Accession NumberBC025782
Gene Symbol CD4
Gene ID (NCBI) 920
RRIDAB_2883024
Conjugate APC Fluorescent Dye
Excitation/Emission Maxima Wavelengths650 nm / 660 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

Protocols

Product Specific Protocols
FC protocol for APC CD4 antibody APC-65143Download protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Macarena Lucia (Verified Customer) (09-28-2022)

Good staining at recommended dilution

  • Applications: Flow Cytometry
  • Cell Tissue Type: primary T cell