FcZero-rAb™ APC Anti-Human CD16 (3G8) Rabbit IgG Recombinant Antibody

CD16 FcZero-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

APC Fluorescent Dye

CloneNo.

3G8

Cat no : APC-FcA65612

Synonyms

Fc gamma RIII alpha, Fc gamma RIII, CD16-II, CD16a antigen, CD16A

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Validation Data Gallery

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  FC experiment of human peripheral blood leukocyte using APC-FcA65612

  1x10^6 human peripheral blood leukocytes were surface stained with 5 ul APC Anti-Human CD16 (3G8) Rabbit IgG Recombinant Antibody (APC-FcA65612, Clone: 3G8), or 5 ul APC Rabbit IgG Isotype Control Recombinant Antibody (APC-FcA98136, Clone: 240953C9). Cells were not fixed.

• 

  FC experiment of human peripheral blood leukocyte using APC-FcA65612

  1x10^6 human peripheral blood leukocytes were surface stained with 5 ul APC Anti-Human CD16 (3G8) Rabbit IgG Recombinant Antibody (APC-FcA65612, Clone:3G8)(red), or 5 ul APC Rabbit IgG Isotype Control Recombinant Antibody (APC-FcA98136, Clone: 240953C9) (blue). Cells were not fixed.

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Tested Applications

• Positive FC detected in: human peripheral blood leukocytes

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

APC-FcA65612 targets CD16 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: N/A
• Full Name: Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
• Calculated Molecular Weight: 254 aa, 29 kDa
• GenBank Accession Number: BC017865
• Gene Symbol: CD16a
• Gene ID (NCBI): 2214
• Conjugate: APC Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 650 nm / 660 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B. Clone 3G8 recognizes both the CD16a and CD16b (PMID: 7592758).