CoraLite® Plus 594 Anti-Human CD155/PVR (SKII.4)

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  FC experiment of U-937 using CL594-65251

  U-937 cells (positive) and Jurkat cells (negative) were mixed 1:1 and 1x10^6 cells were surface stained with 5 ul CoraLite® Plus 594 Anti-Human CD155/PVR (CL594-65251, Clone: SKII.4) (red) or CoraLite®594 Mouse IgG1 Isotype Control (CL594-65124, Clone: MOPC-21) (blue). Cells were not fixed.

• 

  FC experiment of U-937 using CL594-65251

  U-937 cells (positive) and Jurkat cells (negative) were mixed 1:1 and 1x10^6 cells were surface stained with 5 ul CoraLite® Plus 594 Anti-Human CD155/PVR (CL594-65251, Clone: SKII.4) or CoraLite®594 Mouse IgG1 Isotype Control (CL594-65124, Clone: MOPC-21). Cells were not fixed.

CD155/PVR Monoclonal Antibody for FC

Cat No. CL594-65251

Clone No.SKII.4

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC

Synonyms

PVR, CD155, HVED, Necl 5, NECL5

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Tested Applications

• Positive FC detected in: U-937 cells

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-65251 targets CD155/PVR in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: SK-N-AS human neuroblastoma cells
• Full Name: poliovirus receptor
• Calculated Molecular Weight: 45 kDa
• GenBank Accession Number: BC015542
• Gene Symbol: CD155/PVR
• Gene ID (NCBI): 5817
• Conjugate: CoraLite® Plus 594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 594 nm / 615 nm
• Form: Liquid
• Purification Method: N/A
• UNIPROT ID: P15151
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD155, also known as PVR, is a type I transmembrane glycoprotein in the immunoglobulin superfamily. It contains three extracellular immunoglobulin-like domains, D1-D3, of which D1 is recognized by the virus. Mature human CD155 consists of a 323 amino acid extracellular domain with one N-terminal V-type and two C2-type Ig-like domains, a 24 amino acid transmembrane segment, and a 50 amino acid cytoplasmic tail. CD155 is thought to play a role in adhesion by interaction with the ECM component vitronectin as well as a role in NK killing of tumor cells. CD155 binds to two receptors of NK cells, CD96 and CD226, and accumulates at cell-cell contact sites, leading to the formation of mature immune synapses between NK cells and target cells. CD155 serves as the entry receptor for poliovirus and thereby mediates human susceptibility to poliovirus infection.