CD147 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

CD147 Monoclonal Antibody for WB, IF, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IF, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G12B5

Cat no : 66443-1-PBS

Synonyms

5F7, Basigin, basigin (Ok blood group), BSG, CD147, Collagenase stimulatory factor, EMMPRIN, HAb18G, M6, OK, OK blood group antigen, TCSF

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Validation Data Gallery

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  WB analysis using 66443-1-Ig (same clone as 66443-1-PBS)

  Untreated and PNGase F-treated lysates of TF-1 cells, MOLT-4 cells, Jurkat cells were subjected to SDS PAGE followed by western blot with 66443-1-Ig (CD147 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66443-1-PBS in a different storage buffer formulation.

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  WB analysis of human spleen using 66443-1-Ig (same clone as 66443-1-PBS)

  human spleen tissue were subjected to SDS PAGE followed by western blot with 66443-1-Ig (CD147 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66443-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 66443-1-Ig (same clone as 66443-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66443-1-Ig (CD147 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66443-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 66443-1-Ig (same clone as 66443-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66443-1-Ig (CD147 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66443-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66443-1-Ig (same clone as 66443-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66443-1-Ig (CD147 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66443-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66443-1-Ig (same clone as 66443-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66443-1-Ig (CD147 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66443-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66443-1-Ig (same clone as 66443-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66443-1-Ig (CD147 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66443-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66443-1-Ig (same clone as 66443-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66443-1-Ig (CD147 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66443-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 66443-1-Ig (same clone as 66443-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD147 antibody (66443-1-Ig, Clone: 1G12B5 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66443-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 66443-1-Ig (same clone as 66443-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD147 antibody (66443-1-Ig, Clone: 1G12B5 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66443-1-PBS in a different storage buffer formulation.

• 

  FC experiment of Jurkat using 66443-1-Ig (same clone as 66443-1-PBS)

  1X10^6 Jurkat cells were stained with 0.20 ug Anti-Human CD147 (66443-1-Ig, Clone:1G12B5) (red) or 0.20 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone:T1F8D3F10) (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000. Cells were fixed with 90% MeOH. This data was developed using the same antibody clone with 66443-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66443-1-PBS targets CD147 in WB, IF, FC, IHC, Indirect ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD147 fusion protein Ag2619
• Full Name: basigin (Ok blood group)
• Calculated Molecular Weight: 385 aa, 42 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC009040
• Gene Symbol: BSG
• Gene ID (NCBI): 682
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD147, also known as Basigin or extracellular matrix metalloproteinase inducer (EMMPRIN), is a transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 7812975). The molecule is composed of an intracellular portion, an extracellular portion and a single transmembrane region. CD147 is expressed on a variety of cell types (e.g., hematopoietic, epithelial, and endothelial cells) and at varying levels (PMID: 32968061). Increased expression of CD147 occurs in many tumors. CD147 is a pleiotropic molecule that plays an important role in fetal, neuronal, lymphocyte and extracellular matrix development (PMID: 17945211). CD147 has been identified as a receptor essential for erythrocyte invasion by Plasmodium falciparum (PMID: 22080952). It has been reported that spike protein of SARS-CoV-2 binds to CD147 on host cells, thereby mediating the viral invasion (Wang, Ke, et al, BioRxiv, 2020). The CD147 gene, designated BSG for basigin, is located on chromosome 19p13.3. Four transcript variants encoding different isoforms have been found (PMID: 21536654). CD147 migrates on SDS-PAGE usually between 35 and 65 kDa, depending on the degree of glycosylation (PMID: 17945211).