Anti-Human CD146 (P1H12)

CD146 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Rabbit, Canine, Mouse, Human and More (1)

Applications

FC

Conjugate

Unconjugated

CloneNo.

P1H12

Cat no : 65181-1-Ig

Synonyms

CD146, MCAM, MUC18



Tested Applications

Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Published Applications

FCSee 2 publications below

Product Information

65181-1-Ig targets CD146 in FC applications and shows reactivity with Rabbit, Canine, Mouse, Human samples.

Tested Reactivity Rabbit, Canine, Mouse, Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen cultured human umbilical cells
Full Name melanoma cell adhesion molecule
Calculated molecular weight 646 aa, 72 kDa
GenBank accession numberBC056418
Gene symbol MCAM
Gene ID (NCBI) 4162
RRIDAB_2881483
Conjugate Unconjugated
Form Liquid
Purification Method Purified by protein-A affinity chromatography
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988).

Protocols

Product Specific Protocols
FC protocol for CD146 antibody 65181-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanFC

Front Cell Dev Biol

Nrf2 Activation Is Involved in Cyclic Mechanical Stress-Stimulated Osteogenic Differentiation in Periodontal Ligament Stem Cells via PI3K/Akt Signaling and HO1-SOD2 Interaction.

Authors - Xun Xi
humanFC

Stem Cell Res Ther

Menstrual blood-derived endometrial stem cells alleviate neuroinflammation by modulating M1/M2 polarization in cell and rat Parkinson's disease models

Authors - Han Li