Anti-Human CD146 (P1H12)

CD146 Monoclonal Antibody for IF/ICC, FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human, mouse, rabbit, canine

Applications

IF/ICC, FC

Conjugate

Unconjugated

Publications

2

CloneNo.

P1H12

Cat no : 65181-1-Ig

Synonyms

S-endo 1 endothelial-associated antigen, P1H12, MUC18, Melanoma-associated antigen MUC18, Melanoma-associated antigen A32

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Validation Data Gallery

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  IF Staining of HUVEC using 65181-1-Ig

  Immunofluorescent analysis of un-fixed HUVEC cells using CD146 antibody (65181-1-Ig, Clone: P1H12 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).

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  FC experiment of HeLa using 65181-1-Ig

  1X10^6 HeLa cells were surface stained with 0.2 ug Anti-Human CD146 (65181-1-Ig, Clone:P1H12) and APC-conjugated Goat Anti-Mouse IgG at dilution 1:1000. Cells were not fixed.

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Tested Applications

• Positive IF/ICC detected in: HUVEC cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 2 publications below

Product Information

65181-1-Ig targets CD146 in IF/ICC, FC applications and shows reactivity with human, mouse, rabbit, canine samples.

• Tested Reactivity: human, mouse, rabbit, canine
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: cultured human umbilical cells
• Full Name: melanoma cell adhesion molecule
• Calculated Molecular Weight: 646 aa, 72 kDa
• GenBank Accession Number: BC056418
• Gene Symbol: CD146
• Gene ID (NCBI): 4162
• RRID: AB_2881483
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Purified by protein-A affinity chromatography
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988).

Publications

Species	Application	Title
human	FC	Front Cell Dev Biol; Nrf2 Activation Is Involved in Cyclic Mechanical Stress-Stimulated Osteogenic Differentiation in Periodontal Ligament Stem Cells via PI3K/Akt Signaling and HO1-SOD2 Interaction.; Authors - Xun Xi
human	FC	Stem Cell Res Ther; Menstrual blood-derived endometrial stem cells alleviate neuroinflammation by modulating M1/M2 polarization in cell and rat Parkinson's disease models; Authors - Han Li