Anti-Human CD146 (P1H12)
CD146 Monoclonal Antibody for FC
Host / Isotype
Mouse / IgG1, kappa
Reactivity
Rabbit, Canine, Mouse, Human and More (1)
Applications
FC
Conjugate
Unconjugated
CloneNo.
P1H12
Cat no : 65181-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive FC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
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This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
FC | See 2 publications below |
Product Information
65181-1-Ig targets CD146 in FC applications and shows reactivity with Rabbit, Canine, Mouse, Human samples.
Tested Reactivity | Rabbit, Canine, Mouse, Human |
Cited Reactivity | human |
Host / Isotype | Mouse / IgG1, kappa |
Class | Monoclonal |
Type | Antibody |
Immunogen | cultured human umbilical cells |
Full Name | melanoma cell adhesion molecule |
Calculated Molecular Weight | 646 aa, 72 kDa |
GenBank Accession Number | BC056418 |
Gene Symbol | CD146 |
Gene ID (NCBI) | 4162 |
RRID | AB_2881483 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Purified by protein-A affinity chromatography |
Storage Buffer | PBS with 0.09% sodium azide. |
Storage Conditions | Store at 2-8°C. Stable for one year after shipment. |
Background Information
CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988).
Protocols
Product Specific Protocols | |
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FC protocol for CD146 antibody 65181-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Front Cell Dev Biol Nrf2 Activation Is Involved in Cyclic Mechanical Stress-Stimulated Osteogenic Differentiation in Periodontal Ligament Stem Cells via PI3K/Akt Signaling and HO1-SOD2 Interaction. | ||
Stem Cell Res Ther Menstrual blood-derived endometrial stem cells alleviate neuroinflammation by modulating M1/M2 polarization in cell and rat Parkinson's disease models |