Anti-Human CD146/MCAM (P1H12)

• 

  IF Staining of HUVEC using 65181-1-Ig

  Immunofluorescent analysis of un-fixed HUVEC cells using CD146 antibody (65181-1-Ig, Clone: P1H12 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).

• 

  FC experiment of HeLa using 65181-1-Ig

  1X10^6 HeLa cells were surface stained with 0.2 ug Anti-Human CD146 (65181-1-Ig, Clone:P1H12) and APC-conjugated Goat Anti-Mouse IgG at dilution 1:1000. Cells were not fixed.

CD146/MCAM Monoclonal Antibody for IF/ICC, FC

Cat No. 65181-1-Ig

Clone No.P1H12

Publications(5)

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human, mouse, rabbit, canine

Applications

WB, IF/ICC, FC

Synonyms

CD146, MCAM, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, MUC18

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Tested Applications

• Positive IF/ICC detected in: HUVEC cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000
• Flow Cytometry (FC): FC : 0.2 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below
• FC: See 3 publications below

Product Information

65181-1-Ig targets CD146/MCAM in WB, IF/ICC, FC applications and shows reactivity with human, mouse, rabbit, canine samples.

• Tested Reactivity: human, mouse, rabbit, canine
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  cultured human umbilical cells

• Full Name: melanoma cell adhesion molecule
• Calculated Molecular Weight: 646 aa, 72 kDa
• GenBank Accession Number: BC056418
• Gene Symbol: CD146
• Gene ID (NCBI): 4162
• RRID: AB_2881483
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Purified by protein-A affinity chromatography
• UNIPROT ID: P43121
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	FC	Front Cell Dev Biol; Nrf2 Activation Is Involved in Cyclic Mechanical Stress-Stimulated Osteogenic Differentiation in Periodontal Ligament Stem Cells via PI3K/Akt Signaling and HO1-SOD2 Interaction.; Authors - Xun Xi
human	FC	Stem Cell Res Ther; Menstrual blood-derived endometrial stem cells alleviate neuroinflammation by modulating M1/M2 polarization in cell and rat Parkinson's disease models; Authors - Han Li
human	IF	J Mol Histol; Effects of simulated microgravity on dental pulp stem cell stemness; Authors - Huailong Hou
human	WB	Front Pharmacol; CD146 promotes resistance of NSCLC brain metastases to pemetrexed via the NF-κB signaling pathway; Authors - Hao Qu
human	FC	Biochim Biophys Acta Mol Cell Res; Senolytic elimination of senescent cells improved periodontal ligament stem cell-based bone regeneration partially through inhibiting YAP; Authors - Linglu Jia