CD14 Recombinant antibody

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  WB analysis of RAW 264.7 using 83297-8-RR

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 83297-8-RR (CD14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of RAW 264.7 using 83297-8-RR

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CD14 antibody (83297-8-RR, Clone: 240225C2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 83297-8-RR

  Biolayer interferometry (BLl) kinetic assays of 83297-8-RR against Mouse CD14 were performed. The affinity constant is 0.219 nM.

CD14 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 83297-8-RR

Clone No.240225C2

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IF/ICC, ELISA

Synonyms

CD14 antigen, 240225C2

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Tested Applications

• Positive WB detected in: RAW 264.7 cells
• Positive IF/ICC detected in: RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83297-8-RR targets CD14 in WB, IF/ICC, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: CD14 antigen
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 45-55 kDa
• GenBank Accession Number: NM_009841.4
• Gene Symbol: Cd14
• Gene ID (NCBI): 12475
• RRID: AB_3670967
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P10810
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.