CoraLite®594-conjugated CD133 Polyclonal antibody

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• KD/KO Validated

CD133 Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-18470

Synonyms

PROM1, MSTP061, MCDR2, CORD12, Antigen AC133

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Validation Data Gallery

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  IF Staining of mouse kidney using CL594-18470

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using CoraLite®594 CD133 antibody (CL594-18470) at dilution of 1:200.

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  IF Staining of mouse kidney using CL594-18470

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using CoraLite®594 CD133 antibody (CL594-18470) at dilution of 1:200.

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Tested Applications

• Positive IF-P detected in: mouse kidney tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-18470 targets CD133 in IF-P applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD133 fusion protein Ag13328
• Full Name: prominin 1
• Calculated Molecular Weight: 97 kDa
• GenBank Accession Number: BC012089
• Gene Symbol: CD133
• Gene ID (NCBI): 8842
• RRID: AB_2919847
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

CD133, also known as PROM1 (prominin-1) or AC133, belongs to the prominin family. CD133 is a transmembrane glycoprotein with an NH2-terminal extracellular domain, five transmembrane loops and a cytoplasmic tail. The expression of CD133 has been reported in hematopoietic stem cells, endothelial progenitor cells, neuronal and glial stem cells, suggesting the potential role of CD133 as a cell surface marker of adult stem cells. CD133 has also been reported as a marker of cancer stem cells in various human tumors.