APC Anti-Human CD13 (WM15)

CD13 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human, Non-human primates

Applications

FC

Conjugate

APC Fluorescent Dye

CloneNo.

WM15

Cat no : APC-65186

Synonyms

Alanyl aminopeptidase, Aminopeptidase M, Aminopeptidase N, ANPEP, AP M, AP N, APN, CD13, gp150, hAPN, LAP1, Microsomal aminopeptidase, p150, PEPN

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Validation Data Gallery

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  FC experiment of whole blood using APC-65186

  Human whole blood was treated with ACK lysis buffer then 1x10^6 cells were surface stained with 5 ul APC Anti-Human CD13 (WM15) (APC-65186) or APC Mouse IgG1 Isotype Control. Cells were not fixed. Granulocytes were gated.

• 

  FC experiment of whole blood using APC-65186

  Human whole blood was treated with ACK lysis buffer then 1x10^6 cells were surface stained with 5 ul APC Anti-Human CD13 (WM15) (APC-65186) or APC Mouse IgG1 Isotype Control. Cells were not fixed. Granulocytes were gated.

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Tested Applications

• Positive FC detected in: human peripheral blood granulocytes

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

APC-65186 targets CD13 in FC applications and shows reactivity with Human, Non-human primates samples.

• Tested Reactivity: Human, Non-human primates
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Human AML cells
• Full Name: alanyl (membrane) aminopeptidase
• Calculated Molecular Weight: 110 kDa
• GenBank Accession Number: BC058928
• Gene Symbol: CD13
• Gene ID (NCBI): 290
• RRID: AB_2883046
• Conjugate: APC Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 650 nm / 660 nm
• Form: Liquid
• Purification Method: The purified antibody is conjugated with allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD13, also named as APN, ANPEP (aminopeptidase N) or PEPN, is a heavily glycosylated, ~150-240 kDa, type-II membrane, expressed by most cells of myeloid origin including monocytes, macrophages, granulocytes, and their hematopoietic precursors. It is also abundantly expressed in the brush border of epithelial cells from renal proximal tubules and small intestine, in prostatic epithelial cells, in bile duct canaliculi, in mast cells, and, in some cases, in fibroblasts and smooth muscle cells. CD13 is a multifunctional protein and plays varying roles in cell migration, cell proliferation, cell differentiation and so on. CD13 participates in angiogenesis generating and modulating angiogenic signals, and can be a marker of angiogenic vessels. CD13 is also a pan-myeloid marker, present on mature granulocytes and monocytes. (PMID: 8805662, 10098327, 18603472, 18097955, 17897790, 17888402, 21339174)