Anti-Human CD13 (WM15)

CD13 Monoclonal Antibody for IF/ICC, FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human, non-human primates

Applications

IF/ICC, FC

Conjugate

Unconjugated

CloneNo.

WM15

Cat no : 65186-1-Ig

Synonyms

APN, AP-M, ANPEP, Aminopeptidase N, Aminopeptidase M

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Validation Data Gallery

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  IF Staining of HUVEC using 65186-1-Ig

  Immunofluorescent analysis of un-fixed HUVEC cells using CD13 antibody (65186-1-Ig, Clone: WM15 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).

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  IF Staining of THP-1 using 65186-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CD13 antibody (65186-1-Ig, Clone: WM15 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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  FC experiment of human PBMCs using 65186-1-Ig

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD13 (65186-1-Ig, Clone:WM15) and FITC-Goat anti-Mouse IgG at dilution 1:1000 or unstained. Cells were not fixed. Monocytes were gated.

• 

  FC experiment of human PBMCs using 65186-1-Ig

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD13 (65186-1-Ig, Clone:WM15) and FITC-Goat anti-Mouse IgG at dilution 1:1000 or unstained. Cells were not fixed. Monocytes were gated.

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Tested Applications

• Positive IF/ICC detected in: HUVEC cells, THP-1 cells
• Positive FC detected in: human PBMCs

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

65186-1-Ig targets CD13 in IF/ICC, FC applications and shows reactivity with human, non-human primates samples.

• Tested Reactivity: human, non-human primates
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Human AML cells
• Full Name: alanyl (membrane) aminopeptidase
• Calculated Molecular Weight: 110 kDa
• GenBank Accession Number: BC058928
• Gene Symbol: CD13
• Gene ID (NCBI): 290
• RRID: AB_2918457
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD13, also named as APN, ANPEP (aminopeptidase N) or PEPN, is belongs to the peptidase M1 family. CD13 is a heavily glycosylated, ~150-240 kDa, type-II membrane, expressed by most cells of myeloid origin including monocytes, macrophages, granulocytes, and their hematopoietic precursors. It is also abundantly expressed in the brush border of epithelial cells from renal proximal tubules and small intestine, in prostatic epithelial cells, in bile duct canaliculi, in mast cells, and, in some cases, in fibroblasts and smooth muscle cells. CD13 is a multifunctional protein and plays varying roles in cell migration, cell proliferation, cell differentiation and so on. CD13 participates in angiogenesis generating and modulating angiogenic signals, and can be a marker of angiogenic vessels. CD13 is also a pan-myeloid marker, present on mature granulocytes and monocytes. (PMID: 8805662, 10098327, 18603472, 18097955, 17897790, 17888402, 21339174)