CoraLite® Plus 647 Anti-Mouse IL-7Ra/CD127 (A7R34)

IL-7Ra/CD127 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

mouse

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

A7R34

Cat no : CL647-65093

Synonyms

CD127, Il7r, Il-7r, IL- 7Ralpha, IL 7Ralpha

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Validation Data Gallery

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  FC experiment of C57BL/6 mouse splenocytes using CL647-65093

  1X10^6 C57BL/6 mouse splenocytes were surface co-stained with FITC Plus Anti-Mouse CD3 (17A2) and 0.5 ug CoraLite® Plus 647 Anti-Mouse IL-7Ra/CD127 (CL647-65093, Clone: A7R34) or 0.5 ug CoraLite® Plus 647 Rat IgG2a Isotype Control (2A3). Cells were not fixed.

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65093 targets IL-7Ra/CD127 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: IL-7Ra-IgG1 fusion protein
• Full Name: interleukin 7 receptor
• GenBank Accession Number: BC089571
• Gene Symbol: CD127
• Gene ID (NCBI): 16197
• RRID: AB_2883677
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD127, also known as IL-7R subunit alpha (IL-7Rα), is a type I membrane glycoprotein expressed on thymocytes, B cell precursors, most T cells, and some lymphoid and myeloid cells (PMID: 8415665). IL-7R is a heterodimer composed of CD127 and IL-2Rγ (CD132), which is a common gamma chain shared by the receptors of various cytokines, including interleukins 2, 4, 7, 9, and 15 (PMID: 8266077; 9862091). IL-7R plays critical roles in lymphocyte development and homeostasis (PMID: 26336149). CD127 can also act as a receptor for thymic stromal lymphopoietin (TSLP) (PMID: 10974032).