CD126/IL-6R alpha Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

CD126/IL-6R alpha Polyclonal Antibody for WB, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 23457-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of Jurkat using 23457-1-AP (same clone as 23457-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23457-1-AP (IL-6R alpha antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23457-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 23457-1-AP (same clone as 23457-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 23457-1-AP (IL-6R alpha antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23457-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse spleen using 23457-1-AP (same clone as 23457-1-PBS)

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 23457-1-AP (IL-6R alpha antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23457-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse spleen using 23457-1-AP (same clone as 23457-1-PBS)

  IP result of anti-IL-6R alpha (IP:23457-1-AP, 4ug; Detection:23457-1-AP 1:500) with mouse spleen tissue lysate 4000ug. This data was developed using the same antibody clone with 23457-1-PBS in a different storage buffer formulation.

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Product Information

23457-1-PBS targets CD126/IL-6R alpha in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD126/IL-6R alpha fusion protein Ag18263
• Full Name: interleukin 6 receptor
• Calculated Molecular Weight: 468 aa, 52 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC132684
• Gene Symbol: IL-6R alpha
• Gene ID (NCBI): 3570
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Interleukin-6 (IL-6) is a pleiotropic cytokine produced by a variety of cells during infection, trauma, and immunological challenge (PMID: 8274730). IL-6 acts via a receptor complex consisting of two distinct membrane-bound glycoproteins, an 80-kDa IL-6-binding subunit (IL-6R alpha, CD126, gp80) and a 130-kDa signal-transducing element (gp130, CD130). After binding of IL-6 to membrane-bound IL-6R alpha, the complex of IL-6 and IL-6R alpha associates with gp130, thus activating the receptor (PMID: 9716487). Expression of gp130 is found in almost all organs while expression of IL-6R alpha is predominantly confined to hepatocytes and leukocyte subpopulations (monocytes, neutrophils, T cells, and B cells) (PMID: 11149892). Soluble form of IL-6R alpha has been found in human serum and urine (PMID: 2529343; 1545125).