FITC Plus Anti-Human CD11c (3.9) Mouse IgG1 Recombinant Antibody

CD11c Recombinant Antibody for FC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC

Conjugate

FITC Plus Fluorescent Dye

CloneNo.

3.9

Cat no : FITC-65511

Synonyms

CD11C, Integrin alpha X, ITGAX, Leu M5, SLEB6

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Validation Data Gallery

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  FC experiment of human PBMCs using FITC-65511

  1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD11c Mouse Recombinant Antibody (FITC-65511, Clone:3.9) or FITC Plus Mouse IgG1 Isotype Control (FITC-65124, Clone: MOPC-21). Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Monocytes were gated.

• 

  FC experiment of human PBMCs using FITC-65511

  1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD11c Mouse Recombinant Antibody (FITC-65511, Clone:3.9) or FITC Plus Mouse IgG1 Isotype Control (FITC-65124, Clone: MOPC-21). Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Monocytes were gated.

• 

  FC experiment of human PBMCs using FITC-65511

  1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD11c Mouse Recombinant Antibody (FITC-65511, Clone:3.9) or 5 ul FITC Plus Anti-Human CD11c Mouse Monoclonal Antibody (FITC-65086, Clone:3.9). Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Monocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

FITC-65511 targets CD11c in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Recombinant
• Type: Antibody
• Immunogen: Rheumatoid synovial cells and human monocytes
• Full Name: integrin, alpha X (complement component 3 receptor 4 subunit)
• Calculated Molecular Weight: 1169 aa, 129 kDa
• GenBank Accession Number: BC038237
• Gene Symbol: ITGAX
• Gene ID (NCBI): 3687
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11c, also known as integrin αX, is a type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, a subset of B cells, NK cells and dendritic cells (PMID: 2897326; 1680915; 1694698; 17389580). As a result of its high level of expression on most dendritic cells, CD11c is typically considered to be a marker of conventional dendritic cells (PMID: 27119555). CD11c forms an α/β heterodimer with CD18 (integrin β2). CD11c/CD18 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis (PMID: 1671533).