Anti-Mouse CD11c (N418) Mouse IgG2a Recombinant Antibody

CD11c Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

Mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

N418

Cat no : 65602-1-MR

Synonyms

AI449405, Cd11c, Cr4, integrin alpha X, Itgax

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Validation Data Gallery

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  FC experiment of mouse splenocytes using 65602-1-MR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD11c (N418) Mouse IgG2a Recombinant Antibody (65602-1-MR, Clone: N418) (right), or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (left) and FITC anti-mouse IgG2a Antibody. Cells well then stained with APC Anti-Mouse MHC Class II (I-A/I-E) (M5/114.15.2) (APC-65122, Clone: M5/114.15.2). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using 65602-1-MR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD11c (N418) Mouse IgG2a Recombinant Antibody (65602-1-MR, Clone: N418) (right), or 0.25 ug Anti-Mouse CD11c (N418) (65130-1-Ig, Clone: N418) (left) and FITC anti-mouse IgG2a Antibody. Cells well then stained with APC Anti-Mouse MHC Class II (I-A/I-E) (M5/114.15.2) (APC-65122, Clone: M5/114.15.2). Cells were not fixed.

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65602-1-MR targets CD11c in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: Mouse spleen dendritic cells
• Full Name: integrin alpha X
• GenBank Accession Number: BC167225
• Gene Symbol: CD11c
• Gene ID (NCBI): 16411
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11c, also known as integrin αX, is a type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, a subset of B cells, NK cells and dendritic cells (PMID: 2897326; 1680915; 1694698; 17389580). As a result of its high level of expression on most dendritic cells, CD11c is typically considered to be a marker of conventional dendritic cells (PMID: 27119555). CD11c forms an α/β heterodimer with CD18 (integrin β2). CD11c/CD18 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis (PMID: 1671533).