Anti-Human CD11c (BU15)

CD11c Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

BU15

Cat no : 65196-1-Ig

Synonyms

CD11C, Integrin alpha X, ITGAX, Leu M5, SLEB6

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Validation Data Gallery

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  FC experiment of human PBMCs using 65196-1-Ig

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD11c (65196-1-Ig, Clone:BU15) and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red) or unstained. Cells were not fixed. Monocytes were gated.

• 

  FC experiment of human PBMCs using 65196-1-Ig

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD11c (65196-1-Ig, Clone:BU15) and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Monocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65196-1-Ig targets CD11c in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Human synovial fluid dendritic cells
• Full Name: integrin, alpha X (complement component 3 receptor 4 subunit)
• Calculated Molecular Weight: 1169 aa, 129 kDa
• GenBank Accession Number: BC038237
• Gene Symbol: CD11c
• Gene ID (NCBI): 3687
• RRID: AB_2918466
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11c, also known as integrin αX, is a type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, a subset of B cells, NK cells and dendritic cells (PMID: 2897326; 1680915; 1694698; 17389580). As a result of its high level of expression on most dendritic cells, CD11c is typically considered to be a marker of conventional dendritic cells (PMID: 27119555). CD11c forms an α/β heterodimer with CD18 (integrin β2). CD11c/CD18 acts as a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis (PMID: 1671533).